endothelial cell surface marker cd31  (PromoCell)

Journal: PLoS ONE

Article Title: Recapitulation of prostate tissue cell type-specific transcriptomes by an in vivo primary prostate tissue xenograft model

doi: 10.1371/journal.pone.0233899

Figure Lengend Snippet: Volcano plot presentation of differential expression of two-way comparisons between cell types isolated from fresh prostate tissue (A-C) and from primary prostate tissue xenografts (D-F). Cell type specific genes identified in this study are highlighted in red, yellow, and blue for endothelial cell-specific (Endo), epithelial cell-specific (Epi), and stromal cell-specific (Stroma), respectively. The horizontal line indicates an adjusted p -value of 0.05. An upward arrow indicates a decrease in adjusted p -value while a downward arrow indicates an increase in adjusted p -value. The two vertical lines indicate a threshold of log 2 fold of difference of -1 (left) or +1 (right). Genes are considered significantly differentially expressed if they lie on or above the horizontal line with log 2 fold of difference ≤ -1or log 2 fold of difference ≥ 1. Percentage values indicate the percentage of the subset of identified cell type specific genes of a particular cell type that were significantly differentially expressed relative to the total number of cell type specific genes for that cell type for both positive log-fold of difference and negative log-fold of difference, on the right and left respectively. In comparisons of endothelial cells the genes CD31 (PECAM), CD34, VWF, and CD200 are indicated while in epithelial comparisons EPCAM, PSA (KLK3), PSMA (FOLH1), and CDH1are indicated.

Article Snippet: A total of 1.5 ml single cell suspension prepared from fresh prostate tissue or prostate tissue xenografts was mixed with magnetic beads conjugated with an antibody specific for the human endothelial cell surface marker CD31 (25μl of stock solution of 4x 10 8 beads per ml) from Thermo Fisher Scientific, and incubated at 4°C for 30 minutes.

Techniques: Quantitative Proteomics, Isolation

Journal: Scientific Reports

Article Title: Hindlimb Ischemia Impairs Endothelial Recovery and Increases Neointimal Proliferation in the Carotid Artery

doi: 10.1038/s41598-017-19136-6

Figure Lengend Snippet: Effects of hindlimb ischemia on carotid artery endothelium. ( a ) Left: Representative sections of carotid arteries immunostained for the specific endothelial cell marker CD31. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) in carotid arteries sections explanted from BI and FL-BI groups at 14 days after balloon injury. Scale bars = 50 µm. Right: Bar graphs represents the percentage of re-endothelializated circumference of the common carotid artery. * P < 0.05 versus BI group, n = 6 for group. ( b ) Schematic model of the experimental setup. ( c ) Expression levels of selected miRNAs in the carotid artery endothelium 14 days from injury. * P < 0.05 versus BI group; n = 6. ( d ) Relative expression of eNOS, VCAM and ICAM mRNA transcripts in carotid artery endothelium 14 days after injury. * P < 0.05 versus BI group; n = 6.

Article Snippet: Endothelial repair in balloon-injured arteries was evaluated at 14 days after vascular injury using a primary antibody against the endothelial cell surface marker CD31 (R&D Systems, catalog #AF3628, 1:50 dilution).

Techniques: Marker, Staining, Expressing

Journal: Scientific Reports

Article Title: Hindlimb Ischemia Impairs Endothelial Recovery and Increases Neointimal Proliferation in the Carotid Artery

doi: 10.1038/s41598-017-19136-6

Figure Lengend Snippet: Systemic delivery of antagomiR-16 promotes endothelial recovery and inhibits neointima formation in the carotid artery of rats with hindlimb ischemia. ( a ) Schematic model of the experimental setup. ( b ) Expression levels of miR-16 in vascular endothelium. Total RNAs were obtained from vascular endothelium of rat carotid artery 14 days after injury. * P < 0.01 versus control group; n = 6. ( c ) Relative expression of RhoGDIα and eNOS mRNA transcripts in vascular endothelium of rat carotid artery 14 days after injury. * P < 0.05 versus control group; n = 6. ( d ) Left: Representative images of Haematoxylin and eosin staining in balloon-injured carotid arteries at 14 days in rats treated with or without Antago-16. Scale bars, 100 µm. Right: Bar graphs represent the morphometric analysis of arterial sections. Neointima /media ratio of arteries in differently treated groups is shown. * P < 0.05 versus NC group; n = 7. ( e ) Left: Representative sections of carotid arteries immunostained for the specific endothelial cell marker CD31. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Carotid arteries were explanted from experimental groups at 14 days after balloon injury. Scale bars = 50 µm. Right: Bar graphs represents the percentage of re-endothelializated circumference of the common carotid artery. * P < 0.05 versus rat treated with antagomir scrambled; n = 6 for group. ( f ) Left: Representative sections of carotid arteries stained for the macrophage (brown) marker CD68. Carotid arteries were explanted from rats at 14 days after balloon injury. Right: Quantitative data derived from arterial sections stained with CD68 positive cells. * P < 0.05 versus control; n = 5.

Article Snippet: Endothelial repair in balloon-injured arteries was evaluated at 14 days after vascular injury using a primary antibody against the endothelial cell surface marker CD31 (R&D Systems, catalog #AF3628, 1:50 dilution).

Techniques: Expressing, Control, Staining, Marker, Derivative Assay